Gubbels Lab

Lou, J., Y. Rezvani, C.D. Keroack, M.T. Duraisingh, K. Zarringhalam, M.-J. Gubbels. Single cell expression and chromatin access of the Toxoplasma gondii lytic cycle identifies AP2XII-8 as an essential controller of a ribosome regulon. BioRXivs https://www.biorxiv.org/content/10.1101/2023.10.06.561197v1.

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Zarringhalam, K., S. Ye, J. Lou, Y. Rezvani, and M.-J. Gubbels. Cell cycle-regulated ApiAP2s and parasite development: The Toxoplasma paradigm. Current Opinion in Microbiology. 76. 102383. https://doi.org/10.1016/j.mib.2023.102383

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Duraisingh, M.T., M.-J. Gubbels and K. Zarringhalam. 2023. A rising tide of parasite transcriptomics propels pathogen biology. PLoS Biology 21(2): e3001997

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Elsworth, B., C.D. Keroack, Y. Rezvani, J.A. Tennessen, S.A. Sack, A.S. Paul, C.K. Moreira, M.-J. Gubbels, K. Zarringhalam, M.T. Duraisingh. 2022. Babesia divergens egress and invasion of host cells is orchestrated by essential and druggable kinases and proteases. BioRXivs https://doi.org/10.1101/2022.02.17.480550.

 

Rezvani, Y., C. Keroack, B. Elsworth, A. Arriojas, M.J. Gubbels, M.T. Duraisingh, and K. Zarringhalam. 2022. Comparative single cell transcriptional atlases of Babesia species reveal conserved and species-specific expression profiles. BioRXivs: https://doi.org/10.1101/2022.02.11.480160. PLoS Biology 20(9):e3001816 PMCID: PMC9531838 c

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Engelberg, K., T.J. Bechtel, C. Michaud, E. Weerapana, and M.-J. Gubbels. 2021 A more complex basal complex: novel components mapped to the Toxoplasma gondii cytokinesis machinery portray an expanded hierarchy of its assembly and function. BioRXivs https://doi.org/10.1101/2021.10.14.464364. Nature Communications 13: 4644 PMCID: PMC9360017

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Nasuhidehnavi, A. Y. Zhao, A. Punetha, A. Hemphill, H. Li, T. Bechtel, T. Rager, B. Xiong, V. Petrou, M.-J. Gubbels, E. Weerapana, G.S. Yap. 2022. A Role for Basigin in Toxoplasma gondii Infection. Infect. Immun. Aug 1; e0020522. doi: 10.1128/iai.00205-22; PMID: 35913173

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Gubbels, M.-J. D.J.P. Ferguson, S. Saha, J. D. Romano, S. Chavan, V.A. Primo, C. Michaud, I. Coppens, K. Engelberg. 2022 Toxoplasma gondii’s basal complex: the other apicomplexan business end is multifunctional. Front Cell Infect Microbiol. 12:882166. doi: 10.3389/fcimb.2022.882166.

 

Primo Jr, V.A., Y. Rezvani, A. Farrell, C.Q. Murphy, J. Lou, A. Vajdi, G.T. Marth, K. Zarringhalam, and M.-J. Gubbels. 2021. Lipid access in the extracellular milieu of Toxoplasma’s lytic cycle drives lab-adaptation by modulating transcriptional programs. mSystems 6(6): e01196-21. doi.org/10.1128/mSystems.01196-21

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Gubbels, M.-J., I. Coppens, M.T. Duraisingh, K. Zarringhalam, and K. Engelberg, K. 2021. The modular circuitry of apicomplexan cell division plasticity (contribution to special issue “Celebrating Diversity: The Many Cell Cycles of Eukaryotic Microbes”). Front Cell Infect Microbiol. 11:670049. PMCID: PMC8072463

 

Tagoe, D.N.A., A.A. Drozda, J. A. Falco, T. J. Bechtel, E. Weerapana, and M.-J. Gubbels. 2021. Ferlins and DOC2 in Toxoplasma microneme, rhoptry and dense granule secretion. Life 11(3), 217. (special issue "Intracellular Trafficking and Organelle Biogenesis in Apicomplexan Parasites“) doi.org/10.3390/life11030217

 

Tagoe, D.N.A., A. A. Drozda, I. Coppens, B.I. Coleman, and M-J. Gubbels. 2020. Toxoplasma ferlin1 is a versatile and dynamic mediator of microneme trafficking and secretion. bioRxiv: https://biorxiv.org/cgi/content/short/2020.04.27.063628v1

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Engelberg K, Chen CT, Bechtel T, Sánchez Guzmán V, Drozda AA, Chavan S, Weerapana E, Gubbels MJ. 2019. The apical annuli of Toxoplasma gondii are composed of coiled-coil and signalling proteins embedded in the inner membrane complex sutures. Cell Microbiol. e13112. doi: 10.1111/cmi.13112. PMID: 31470470

 

Gubbels, M.-J., and N. Morrissette. 2019. The Toxoplasma Cytoskeleton: Structures, Proteins and Processes (chapter 16). In: Toxoplasma gondii: The Model Apicomplexan, 3rd edition. Eds.: L.M. Weiss and K. Kim. Elsevier Academic Press. Submitted

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Chen, C.-T. and M.-J. Gubbels. 2019. TgCep250 is dynamically processed through the division cycle and essential for structural integrity of the Toxoplasma centrosome. MBoC 30:1160-1169. PMID: 30865554

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Coleman, B.I., S. Saha, S. Sato, K. Engelberg, D.J.P. Ferguson, I Coppens, M. Lodoen, and M.-J. Gubbels. 2018. A member of the ferlin calcium sensor family is essential for Toxoplasma gondii rhoptry secretion. mBio. 9: e01510-18. PMCID: PMC6168857

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Berry, L., C.-T. Chen, M.E. Francia, A. Guerin, A. Graindorge, J.-M. Saliou, M. Grandmougin, S. Wein, C. Bechara, J. Morlon-Guyot, Y. Bordat, M.-J. Gubbels, M. Lebrun, J.-F. Dubremetz and W. Daher. 2018 Toxoplasma gondii chromosomal passenger complex is essential for the organization of a functional mitotic spindle, a prerequisite for productive endodyogeny. Cell Molec Life Sci. 75:4417-4443 PMCID: PMC6260807

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Dubey, R., B. Harrison, S. Dangoudoubiyam, G. Bandini, K. Cheng, A. Kosber, C. Agop Nersesian, D. Howe, J. Samuelson, D.J.P. Ferguson, M.-J. Gubbels. 2017. Differential roles for IMC proteins across Toxoplasma gondii and Sarcocystis neurona development. mSphere 2(5). pii: e00409-17. doi: 10.1128/mSphere.00409-17.

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Saha, S., B.I. Coleman, R. Dubey, I.J. Blader, and M.-J. Gubbels. 2017. Two phosphoglucomutase paralogs facilitate ionophore-triggered secretion of the Toxoplasma micronemes. mSphere. 2: e00521-17.

 

Dubey, R., B.L. Staker, I.T. Foe, M. Bogyo, P.J. Myler, H.M. Ngô, and M.-J. Gubbels. 2017. Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1. Mol. Microbiol. 103:618-634

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Weidner, J.M., S. Kanatani, H. Uchtenhangen, M. Varas-Godoy, T. Schulte, K. Engelberg, M.-J. Gubbels, H. Song Sun, R. Harrison, A. Achour, and A. Barragan. 2016. Toxoplasma gondii 14-3-3 protein is linked to a hypermigratory phenotype in parasitized immune cells. Cell. Microbiol. 18:1537-1550. 

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Carvalho, T.G., L. Berry, L. Reininger, C.-T. Chen, H. El Hajj, M. Bird, O. Kleifeld, S. Hermann, J. Morlon-Guyot, Y. Bordat, M. Lebrun, M.-J. Gubbels, C. Doerig, and W. Daher. 2016. The conserved apicomplexan Aurora kinase 3 is involved in endodyogeny, duplication rate and parasite virulence. Cell. Microbiol. 18:1106-1120 

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Engelberg, K., F.D. Ivey, A. Lin, M. Kono, A. Lorestani, D. Faugno-Fusci, T.W. Gilberger, M. White, and M.-J. Gubbels. 2016.  A MORN1-assocatiated HAD phosphatase in the basal complex is essential for Toxoplasma gondii daughter budding. Cell. Microbiol. 18:1153-1171
 

Skariah, S., O. Walwyn, K. Engelberg, M.-J. Gubbels, N. Kim, B. Lynch, C. Gaylets, A. Ali Sultan, and D. Mordue. 2016.  Toxoplasma gondii’s FIKK kinase is not essential for the parasite’s lytic cycle . Int. J. Parasitol.  46:323-332  

 

Chen, C.-T., M. Farrell, J. de Leon, B. Nwagbara, P. Ebbert, D.J.P. Ferguson, L.A. Lowery, N. Morrissette, and M.-J. Gubbels. 2015. Compartmentalized Toxoplasma EB1 bundles spindle microtubules to secure accurate chromosome segregation. MBoC. 26:4562-4576  

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Blader, I.J., B.I. Coleman, C.-T. Chen, and M.-J. Gubbels. 2015. The Toxoplasma gondii lytic cycle. Ann Rev Microbiol. 69:483-485  

 

Paul, A.S., S., Saha, K. Engelberg, R.Y. Jiang, B.I. Coleman, A.L. Kosber, C.-T. Chen, M. Ganter, N. Espy, T.-W. Gilberger, M.-J. Gubbels#, and M.T. Duraisingh#. 2015. Apicomplexan calcineurin regulates specific attachment of free parasites to host cells for infection. Cell Host Microbe. 18, 1-12  

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Chen, C.-T., and M.-J. Gubbels. 2015. Apicomplexan cell cycle flexibility: centrosome controls the clutch. Trends Parasitol. 31:229-230  

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Brown, K.M., E. Suvorova, A. Farrell, A. McLain, A. Dittmar, G.B. Wiley, G.T. Marth, P.M. Gaffney, M.-J. Gubbels, M. White, and I.J. Blader. . 2014. Forward genetic screening identifies a small molecule that blocks Toxoplasma growth by inhibiting both host- and parasite-encoded kinases PLoS Path. 10(6): e1004180  

Farrell, A., B.I. Coleman, B. Benenati, K.M. Brown, I. Blader, G.T. Marth, and M.-J. Gubbels. 2014. Whole genome profiling of spontaneous and chemical mutagenesis induced mutations in Toxoplasma gondii. BMC Genomics. 15: 354.
 

 Farrell, M., and M.-J. Gubbels 2014. TheToxoplasma gondii kinetochore is required for centrosome association with the centrocone (spindle pole). Cellular Microbiology, 16: 78-94  

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Morlon-Guyot, J., L. Berry, C.-T. Chen, M.-J. Gubbels, M. Lebrun, and W. Daher. 2014. The Toxoplasma gondii Calcium Dependent Protein Kinase 7 is involved in early steps of parasite division and crucial for parasite survival. Cellular Microbiology, 16: 95-114

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Gubbels, M.-J., and Morrissette, N. 2013. The Cytoskeleton: structures and motility (chapter 13). In: Toxoplasma gondii: The Model Apicomplexan, 2nd edition. Eds.: L.M. Weiss and K. Kim. Elsevier Academic Press. ISBN: 9780123964816

 

Yang, N., A. Farrell, W. Niedelman, M. Melo, D. Lu, L. Julien, G.T. Marth, M.-J. Gubbels, and J.P.J. Saeij. 2013. Genetic basis for phenotypic differences between different Toxoplasma  Type I strains. BMC Genomics, 14:467
 

Chen, C.-T., and M.-J. Gubbels. 2013. The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant. J. Cell Sci. 126:3344-3355.
 

Gubbels, M.-J., and M.D. Duraisingh. 2012. Evolution of apicomplexan secretory organelles. Int. J. Parasitol., 42:1071-1081 (invited review)  

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Lorestani, A., F.D. Ivey, S. Thirugnanam, M.A. Busby, G.T. Marth, I. Cheeseman, and M.-J. Gubbels. 2012. Targeted proteomic dissection of Toxoplasma, cytoskeleton sub-compartments using MORN1. Cytoskeleton, 69:1069-1085  

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Bushkin, G.G., P. Magnelli, E. Motari, M.-J. Gubbels, J.P. Dubey, K. Miska, E. Bullitt, C.E. Costello, P.W. Robbins, and J. Samuelson. 2012. β-1,3-glucan, a druggable target, is part of a trabecular scaffold in inner layer of the oocyst wall of Toxoplasma and Eimeria. mBio. 3(5). pii:e00258-12  

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Anderson-White, B.R., J.R. Beck, C.-T. Chen, M. Meissner, P.J. Bradley, and M.-J. Gubbels. 2012. Cytoskeleton development of Toxoplasma gondii. Int. Rev. Cell Mol. Biol. 298:1-31. (invited review) 

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Fung, C., J.R. Beck, S.D. Robertson, M.-J. Gubbels, and P.J. Bradley. 2012. Toxoplasma ISP4 is a central IMC sub-compartment protein whose localization depends on palmitoylation but not myristoylation. Mol. Biochem. Parasitol. 184:99-108. PMCID: PMC3383393  

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Szatanek, T., B.R. Anderson-White, D. Faugno-Fusci, M. White, J.P.J. Saeij, and M.-J. Gubbels. 2012. Cactin is essential for G1 progression of Toxoplasma gondii. Mol. Micro. 84:566-577. PMCID: PMC3331927. 

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Coleman, B.I., and M.-J. Gubbels. 2012. A genetic screen to isolate Toxoplasma gondii host cell egress mutants. J. Vis. Exp. 60:pii3807  

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Farrell, A*. S. Thirugnanam*, A. Lorestani*, J.D. Dvorin*, K.P. Eidell, B.R. Anderson-White, D.J.P. Ferguson, M.T. Duraisingh#, G.T. Marth#, and M.-J. Gubbels#. 2012. A DOC2 protein identified by mutational profiling is essential for apicomplexan parasite exocytosis. Science. 335:218-221  

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Jammallo*, L., K. Eidell*, P.H. Davis, F.J. Dufort, C. Cronin, S. Thirugnanam, T.C. Chiles, D.S. Roos, and M.-J. Gubbels. 2011. An insertional trap for conditional gene expression in Toxoplasma gondii: identification of TAF250 as an essential gene. Mol. Biochem. Parasitol. 175:133-143.  

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Anderson-White, B.R., F.D. Ivey, K, K. Cheng, T. Szatanek, A. Lorestani, C.J. Beckers, D.J.P. Ferguson, N. Sahoo, and M.-J. Gubbels. 2011. A family of intermediate filament-like proteins is sequentially assembled into the cytoskeletal scaffold of Toxoplasma gondii. Cell Microbiol.13:18-31. 

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Lorestani, A., L. Sheiner, K. Yang, S.D. Robertson, N. Sahoo, C.F. Brooks, D.P.J. Ferguson, B. Striepen, and M.-J. Gubbels. 2010. A Toxoplasma MORN1 null mutant is defective in basal complex assembly and apicoplast division. PLoS ONE. 5:e12302  

 

Eidell, K., T. Burke, and M.-J. Gubbels. 2010. Development of a screen to dissect Toxoplasma gondii egress. Mol Biochem Parasitol. 171:97-103  

 

Wilson, D.C., G.M. Grotenbreg, K.N. Liu, Y. Zhao, E.-M. Frickel,  M.-J. Gubbels, H.L. Ploegh, and G.S. Yap. 2010. Differential regulation of effector- and central-memory responses to Toxoplasma gondii infection by IL-12 revealed by tracking of Tgd057-specific CD8+ T cells. PLoS Pathogens, 19:e1000815.  

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Bushkin, G.G., D.M. Ratner, J. Cui, S. Banerjee, M.T. Duraisingh, C.V. Jennings, J.D. Dvorin, M.-J. Gubbels, S.D. Robertson, M. Steffen, B.R. O’Keefe, P.W. Robbins, and J. Samuelson. 2010. Evidence for Darwinian selection against asparagine-linked glycans of Plasmodium and Toxoplasma. Euk. Cell. 9:228-241.  

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Frickel. E.M., N. Sahoo, J. Hopp, M.-J. Gubbels, M.P. Craver, L.J. Knoll, H. Ploegh, and G. Grotebreg. 2008. Parasite stage-specific recognition of endogenous Toxoplasma gondii epitopes by CD8+ T cells. J Inf. Dis. 98:1625-1633 .  

 

Gubbels M.-J., M. White and T. Szatanek. 2008. The cell cycle and Toxoplasma gondii cell division: Tightly knit or loosely stitched? Int. J. Parasitol. 38:1343-1358. (review) 

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Muskavitch, M., N. Barteneva and M.-J. Gubbels. 2008. Chemogenomics and Parasitology: small molecules and cell-based assays to study infectious processes. Comb. Chem. HTS. 11:626-649. (review)  

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Ferguson, D.J.P., N. Sahoo, R.A. Pinches, J.M. Bumstead, F.M. Tomley, and M.-J. Gubbels. 2008. MORN1 has a conserved role in asexual and sexual development across the Apicomplexa. Euk. Cell. 7(4):698-711.  

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Gubbels, M.-J., C.F. Brooks, M. Muthalagi, T. Szatanek, J. Flynn, B. Parrot, B. Striepen and M.W. White. 2008. Forward genetic analysis of the apicomplexan cell and division cycle in Toxoplasma gondii. PLoS Pathogens, 4(2):e36.

 

Frickel, E.-M., V. Quesada, L. Muething, M.-J. Gubbels, E. Spooner, H. Ploegh and K. Artavanis-Tsakonas. 2007. UCHL3 retains dual specificity for ubiquitin and Nedd8 throughout evolution. Cell. Microbiol. 9:1601-1610.

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